RAD18 RAD18 E3 ubiquitin protein ligase [Homo sapiens (human)] - Gene

Summary

Official Symbol: RAD18provided by HGNC
Official Full Name: RAD18 E3 ubiquitin protein ligaseprovided by HGNC
Primary source: HGNC:HGNC:18278
See related: Ensembl:ENSG00000070950 MIM:605256; AllianceGenome:HGNC:18278
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: RNF73
Summary: The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq, Jul 2008]
Expression: Ubiquitous expression in duodenum (RPKM 3.3), lymph node (RPKM 2.7) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 3p25.3

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2023_10	current	GRCh38.p14 (GCF_000001405.40)	3	NC_000003.12 (8877075..8963472, complement)
RS_2023_10	current	T2T-CHM13v2.0 (GCF_009914755.1)	3	NC_060927.1 (8868283..8960543, complement)
105.20220307	previous assembly	GRCh37.p13 (GCF_000001405.25)	3	NC_000003.11 (8918759..9005156, complement)

Chromosome 3 - NC_000003.12 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Implication of E3 ligase RAD18 in UV-induced mutagenesis in human induced pluripotent stem cells and neuronal progenitor cells.
Title: Implication of E3 ligase RAD18 in UV-induced mutagenesis in human induced pluripotent stem cells and neuronal progenitor cells.
Analysis of the Mechanism of RAD18 in Glioma.
Title: Analysis of the Mechanism of RAD18 in Glioma.
RAD18 opposes transcription-associated genome instability through FANCD2 recruitment.
Title: RAD18 opposes transcription-associated genome instability through FANCD2 recruitment.
RAD18 confers radioresistance of esophagus squamous cell carcinoma through regulating p-DNA-PKcs.
Title: RAD18 confers radioresistance of esophagus squamous cell carcinoma through regulating p-DNA-PKcs.
Genome-wide CRISPR screen identified Rad18 as a determinant of doxorubicin sensitivity in osteosarcoma.
Title: Genome-wide CRISPR screen identified Rad18 as a determinant of doxorubicin sensitivity in osteosarcoma.
UBE2B promotes ovarian cancer growth via promoting RAD18 mediated ZMYM2 monoubiquitination and stabilization.
Title: UBE2B promotes ovarian cancer growth via promoting RAD18 mediated ZMYM2 monoubiquitination and stabilization.
REV1 promotes lung tumorigenesis by activating the Rad18/SERTAD2 axis.
Title: REV1 promotes lung tumorigenesis by activating the Rad18/SERTAD2 axis.
RAD18 mediates DNA double-strand break-induced ubiquitination of chromatin protein.
Title: RAD18 mediates DNA double-strand break-induced ubiquitination of chromatin protein.
Translesion Synthesis or Repair by Specialized DNA Polymerases Limits Excessive Genomic Instability upon Replication Stress.
Title: Translesion Synthesis or Repair by Specialized DNA Polymerases Limits Excessive Genomic Instability upon Replication Stress.
PCNA Monoubiquitination Is Regulated by Diffusion of Rad6/Rad18 Complexes along RPA Filaments.
Title: PCNA Monoubiquitination Is Regulated by Diffusion of Rad6/Rad18 Complexes along RPA Filaments.

Submit: New GeneRIF Correction See all GeneRIFs (82)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein	Gene	Interaction	Pubs
integrase	gag-pol	HIV-1 Integrase interacts with (co-immunoprecipitates) and co-localizes with hRad18 suggesting a role for hRad18 in the DNA repair events associated with the HIV-1 integration process	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH12770 (e-PCR)
- UniSTS:20853

SHGC-76680 (e-PCR)
- UniSTS:6808

SHGC-78042 (e-PCR)
- UniSTS:101573

PMC16647P1 (e-PCR)
- UniSTS:271598

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables Y-form DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables damaged DNA binding	NAS Non-traceable Author Statement more info	PubMed
enables identical protein binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables polyubiquitin modification-dependent protein binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-containing complex binding	IPI Inferred from Physical Interaction more info	PubMed
enables single-stranded DNA binding	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA damage response	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA damage response	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA repair	NAS Non-traceable Author Statement more info	PubMed
involved_in positive regulation of chromosome segregation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in postreplication repair	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within protein autoubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein monoubiquitination	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of Rad6-Rad18 complex	IBA Inferred from Biological aspect of Ancestor more info
located_in centrosome	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in nuclear body	IDA Inferred from Direct Assay more info
located_in nuclear inclusion body	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	IDA Inferred from Direct Assay more info
located_in nucleoplasm	TAS Traceable Author Statement more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	NAS Non-traceable Author Statement more info	PubMed
located_in replication fork	IDA Inferred from Direct Assay more info	PubMed
located_in site of double-strand break	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: E3 ubiquitin-protein ligase RAD18

Names: RAD18 homolog; RAD18, S. cerevisiae, homolog; RING finger protein 73; RING-type E3 ubiquitin transferase RAD18; hHR18; hRAD18; postreplication repair protein RAD18; postreplication repair protein hRAD18p

NP_064550.3

EC 2.3.2.27

XP_016862362.1

EC 2.3.2.27

XP_054203240.1

EC 2.3.2.27

XP_054203241.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_020165.4 → NP_064550.3 E3 ubiquitin-protein ligase RAD18

See identical proteins and their annotated locations for NP_064550.3

Status: REVIEWED

Source sequence(s)

AC008151, AC034186, AK023075, DW433240

Consensus CDS

CCDS2571.1

UniProtKB/Swiss-Prot

Q58F55, Q9NRT6, Q9NS91

UniProtKB/TrEMBL

Q53H10

Related

ENSP00000264926.2, ENST00000264926.7

Conserved Domains (4) summary

smart00734
Location:201 → 224

ZnF_Rad18; Rad18-like CCHC zinc finger

cd00162
Location:25 → 63

RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...

TIGR00599
Location:13 → 363

rad18; DNA repair protein rad18

pfam02037
Location:248 → 282

SAP; SAP domain